James Cook University 2 articles published in JoVE Immunology and Infection Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis Laura A. Brannelly1,2, Alexandra A. Roberts1, Lee F. Skerratt1, Lee Berger1 1One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, 2Department of Biological Sciences, University of Pittsburgh We quantify epidermal cell death in frogs with chytridiomycosis using two methods. First, we use terminal transferase-mediated dUTP nick end-labelling (TUNEL) in situ histology to determine differences between clinically infected and uninfected animals. Second, we conduct a time series analysis of apoptosis over infection using a caspase 3/7 protein analysis. Immunology and Infection Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians Laura A. Brannelly1 1School of Public Health, Tropical Medicine and Rehabilitation Sciences, James Cook University Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment protocol.
