2 articles published in JoVE
Analysis of Epididymal Protein Synthesis and Secretion Wei Zhou1,2, Petra Sipilä3, Geoffry N. De Iuliis1,2, Matthew D. Dun2,4, Brett Nixon1,2 1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle Here, we report the immunofluorescence localization of dynamin to illustrate the protocols for the detection of proteins in paraffin-embedded mouse epididymal sections and those of an immortalized epididymal cell line (mECap18). We also describe the protocols for the isolation of secretory proteins from both epididymal fluid and conditioned cell media.
Comparison of Three Different Methods for Determining Cell Proliferation in Breast Cancer Cell Lines Brianna C. Morten1,2, Rodney J. Scott1,2,3, Kelly A. Avery-Kiejda1,2 1Medical Genetics, Hunter Medical Research Institute, 2Priority Research Centre for Cancer, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, 3Pathology North, John Hunter Hospital This protocol describes the use of three different methods for analyzing cell proliferation in breast cancer cell lines. This includes the use of conventional cell counting, luminescence-based cell viability, and cell counting through the use of a cell imager. Each offers advantages for the reproducible measurement of cell proliferation.