3 articles published in JoVE
Induction of Complete Transection-Type Spinal Cord Injury in Mice Ronak Reshamwala1,2,3, Tanja Eindorf2,3, Megha Shah2,3, Graham Smyth2,3, Todd Shelper2,3, James St. John*1,2,3, Jenny Ekberg*2,3 1Griffith Institute for Drug Discovery, Griffith University, 2Menzies Health Institute Queensland, Griffith University, 3Clem Jones Centre for Neurobiology and Stem Cell Research, Griffith University This protocol describes how to create a precise laminectomy for induction of stable transection-type spinal cord injury in the mouse model, with minimal collateral damage for spinal cord injury research.
Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology Michael A. Pearen*1, Hong Kiat Lim*1, Francis D. Gratte2,3, Manuel A. Fernandez-Rojo1,4,5, Sujeevi K. Nawaratna6, Geoffrey N. Gobert7, John K. Olynyk8,9, Janina E. E. Tirnitz-Parker2,10, Grant A. Ramm1,4 1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7 This protocol provides a simple and reliable method for the production of viable precision-cut liver slices from mice. The ex vivo tissue samples can be maintained under laboratory tissue culture conditions for multiple days, providing a flexible model to examine liver pathobiology.
Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells Hannah C. Leeson1,2, Tailoi Chan-Ling3,4, Michael D. Lovelace3,5,6, Jeremy D. Brownlie7, Michael W. Weible II*1,4,7, Ben J. Gu*8 1Griffith Institute for Drug Discovery, Griffith University, 2Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 3Discipline of Anatomy and Histology, School of Medical Science, University of Sydney, 4Bosch Institute, University of Sydney, 5 Providing single-cell sensitivity, real-time flow cytometry is uniquely suited to quantify multimodal receptor functions of live cultures. Using adult neural progenitor cells, the P2X7 receptor function was assessed via calcium influx detected by calcium indicator dye, transmembrane pore formation by ethidium bromide uptake, and phagocytosis using fluorescent latex beads.