Queensland Eye Institute 1 article published in JoVE Bioengineering Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes Jennifer Walshe1, Najla Al Khaled Abdulsalam2, Shuko Suzuki1, Traian V. Chirila1,3,4,5,6,7,8, Damien G. Harkin1,3,4 1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia This protocol describes the critical steps required to establish and grow corneal endothelial cell cultures from explants of human or sheep tissue. A method for subculturing corneal endothelial cells on membranous biomaterials is also presented.
