Department of Primary Industries and Regional Development 1 article published in JoVE Biochemistry Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain Hayley Abbiss1,2,3, Joel P. A. Gummer1,4, Michael Francki5,6, Robert D. Trengove1 1Research and Innovation, Murdoch University, 2Centre for Digital Agriculture, Curtin University, 3Centre for Integrative Metabolomics and Computational Biology, School of Science, Edith Cowan University, 4Centre for Computational and System Medicine, Health Futures Institute, Murdoch University, 5Department of Primary Industries and Regional Development, 6State Agricultural Biotechnology Centre, Murdoch University A method for the untargeted analysis of wheat grain metabolites and lipids is presented. The protocol includes an acetonitrile metabolite extraction method and reversed phase liquid chromatography-mass spectrometry methodology, with acquisition in positive and negative electrospray ionization modes.
