2 articles published in JoVE
Measuring the 50% Haemolytic Complement (CH50) Activity of Serum Maurizio Costabile1 1School of Pharmacy and Medical Sciences, University of South Australia The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of results.
Determining the Reactivity and Titre of Serum using a Haemagglutination Assay Maurizio Costabile1 1School of Pharmacy and Medical Sciences, University of South Australia Haemagglutination is a form of agglutination where antibodies bind to red blood cells. Red blood cells are both readily available and the results are readily observable using the naked eye. This video demonstrates the steps involved in a haemagglutination assay, interpreting the results and determining the titre.