International Atomic Energy Agency 1 article published in JoVE Immunology and Infection Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells Fatima Liaqat1, Richard Thiga Kangethe1, Rudolf Pichler1, Bo Liu1, Johann Huber2, Viskam Wijewardana1, Giovanni Cattoli1, Luca Porfiri1 1Animal Production and Health Section, Joint Food and Agriculture Organization (FAO)/International Atomic Energy Agency (IAEA) Centre of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, 2Teaching and Research Farm Kremesberg, Clinical Unit for Herd Health Management in Ruminants, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna The methodology describes the generation of bovine monocyte-derived dendritic cells (MoDCs) and their application for the in vitro evaluation of antigenic candidates during the development of potential veterinary vaccines in cattle.