VIB BioImaging Core 1 article published in JoVE Biology Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy Christopher J. Guérin1,2,3, Anna Kremer1,2,3, Peter Borghgraef1,2,3, Saskia Lippens1,2,3 1VIB Bio Imaging Core, 2VIB Inflammation Research Center, 3Department of Molecular Biomedical Research, UGent Here, we present a protocol for efficiently combining serial block face and focused ion beam scanning electron microscopy to target an area of interest. This allows for efficient searching, in three dimensions, and locating rare events in a large field of view.
