VIB-KU Leuven Center for Microbiology 1 article published in JoVE Immunology and Infection Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging Noémie Luyts1, Greetje Vande Velde2, Matthias Vanneste1, Helene De Bruyn1, Annelies Janssens1, Natalie Verstraeten3, Thomas Voets1, Wouter Everaerts4 1Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine, KU Leuven, 2Biomedical MRI, Department of Imaging & Pathology, KU Leuven, 3Belgium & KU Leuven Centre of Microbial and Plant Genetics, VIB-KU Leuven Center for Microbiology, Leuven, 4Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven This manuscript describes the intravesical administration of uropathogenic bacteria with a lux operon to induce a urinary tract infection in mice and subsequent longitudinal in vivo analysis of the bacterial load using bioluminescence imaging.