VIB-UGent Center for Inflammation Research 1 article published in JoVE Immunology and Infection Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells Josephine Nemegeer1,2, Kelly Lemeire1,2, Peter Vandenabeele1,2, Jonathan Maelfait1,2 1VIB-UGent Center for Inflammation Research, 2Department of Biomedical Molecular Biology, Ghent University Tyramide signal amplification during immunofluorescent staining enables the sensitive detection of phosphorylated RIPK3 and MLKL during ZBP1-induced necroptosis after HSV-1 infection.
