National Centre of Competence in Research 1 article published in JoVE Biology En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries Yi Yu1,2, Yuyan Xiong1,2, Jean-Pierre Montani2,3, Zhihong Yang1,2, Xiu-Fen Ming1,2 1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, 2Kidney Control of Homeostasis, Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg In this article we describe an adapted relatively easy method using the fluorescence dye diaminofluorescein-2 diacetate (DAF-2DA) and dihydroethidium (DHE) for en face simultaneous detection and visualization of intracellular nitric oxide (NO) and superoxide anion (O2.−) respectively, in freshly isolated intact aortas of an obesity mouse model.
