Centre Suisse d'Electronique et de Microtechnique 1 article published in JoVE Environment A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants Sarah Heub1,2, Noe Tscharner1, Florian Kehl1,3, Petra S. Dittrich2, Stéphane Follonier1, Laurent Barbe1 1 A protocol for the extraction and pre-concentration of estradiol from water samples by using an automated and miniaturized system is presented.