Federal Office for Agriculture 1 article published in JoVE Environment A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci Simon Blaser1,2,3, Hanspeter Diem4, Andreas von Felten4, Morgan Gueuning1, Michael Andreou5, Neil Boonham6,7, Jennifer Tomlinson6, Pie Müller2,3, Jürg Utzinger2,3, Beatrice Frey1, Jürg E. Frey1, Andreas Bühlmann8 1Department of Method Development and Analytics, Agroscope, 2Swiss Tropical and Public Health Institute, 3University of Basel, 4Swiss Federal Plant Protection Service, Federal Office for Agriculture, 5OptiGene Limited, 6Fera Science Limited, 7School of Natural and Environmental Sciences, Newcastle University, 8Department of Plants and Plant Products, Agroscope This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.
