University Hospital Center and University of Lausanne 1 article published in JoVE Immunology and Infection Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches Nicolas Jacquier1, Sébastien Aeby1, Julia Lienard1, Gilbert Greub1 1Institute of Microbiology, University Hospital Center and University of Lausanne Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.
