University of Zurich and University Hospital Zurich 1 article published in JoVE Cancer Research Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research Giovanna Azzarito1, Marta Ewa Szutkowska1, Annalisa Saltari2, Edwin K. Jackson3, Brigitte Leeners1, Marinella Rosselli1, Raghvendra K. Dubey1,3 1Department of Reproductive Endocrinology, Wagistrasse 14, University of Zurich and University Hospital Zurich, 2Department of Dermatology, Wagistrasse 18, University of Zurich and University Hospital Zurich, 3Department of Pharmacology and Chemical Biology, University of Pittsburgh Crosstalk between mammary epithelial cells and endothelial cells importantly contributes to breast cancer progression, tumor growth, and metastasis. In this study, spheroids have been made from breast cancer cells together with vascular and/or lymphatic endothelial cells and demonstrate their applicability as an in vitro system for breast cancer research.
