Palacky University Olomouc 6 articles published in JoVE Environment Mistletoe Eradicator - A Novel Tool for Simultaneous Mechanical and Chemical Control of Mistletoe Khurshid Ahmad Bhat1, Sabina Akhtar2, Naseer Ahmad Dar3, Mohammad Iqbal Bhat3, Farooq Ahmad Bhat1, Rashid Rizwan4, Oleksii Horielov5, Yuliya Krasylenko6 1Department of Plant Pathology, FOA Wadura Sopore, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, 2American University in the Emirates Dubai, 3Krishi Vigyan Kendra, Budgam, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, 4Department of Vegetable Science, FoH, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, 5Department of Dendrology, M.M. Gryshko National Botanical Garden, National Academy of Sciences of Ukraine, 6Department of Biotechnology, Palacký University Olomouc The protocol describes an easy-to-handle tool called Mistletoe Eradicator which combines mechanical and chemical approaches and is efficient in the control of mistletoe infestation on the host trees. The manuscript uses the European mistletoe (Viscum album subsp. album L.) as an example. Behavior Probing the Limits of Egg Recognition Using Egg Rejection Experiments Along Phenotypic Gradients Lindsay Canniff1, Miri Dainson2, Analía V. López3, Mark E. Hauber2, Tomáš Grim4, Peter Samaš5, Daniel Hanley1 1Department of Biology, Long Island University-Post, 2Department of Animal Biology, School of Integrative Biology, University of Illinois, 3Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 4Department of Zoology and Laboratory of Ornithology, Palacký University, 5Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic This protocol provides guidelines for running egg rejection experiments: outlining techniques for painting experimental egg models to emulate the colors of natural bird eggs, conducting fieldwork, and analyzing the collected data. This protocol provides a uniform method for conducting comparable egg rejection experiments. Engineering Methods of Ex Situ and In Situ Investigations of Structural Transformations: The Case of Crystallization of Metallic Glasses Marcel B. Miglierini1,2, Vít Procházka3, Vlastimil Vrba3, Peter Švec4, Dušan Janičkovič4, Peter Matúš5 1Institute of Nuclear and Physical Engineering, Slovak University of Technology in Bratislava, Slovakia, 2Department of Nuclear Reactors, Czech Technical University in Prague, Czech Republic, 3Department of Experimental Physics, Palacky University Olomouc, Czech Republic, 4Institute of Physics, Slovak Academy of Sciences, Bratislava, Slovakia, 5Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University in Bratislava, Slovakia Here, we present a protocol to describe ex situ and in situ investigations of structural transformations in metallic glasses. We employed nuclear-based analytical methods which inspect hyperfine interactions. We demonstrate the applicability of Mössbauer spectrometry and nuclear forward scattering of synchrotron radiation during temperature-driven experiments. Medicine Muscle Imbalances: Testing and Training Functional Eccentric Hamstring Strength in Athletic Populations Petr Stastny1, Michal Lehnert2, James J. Tufano1 1Faculty of Physical Education and Sport, Charles University, 2Faculty of Physical Culture, Palacky University Olomouc The hamstrings are a group of muscles that are sometimes problematic for athletes, resulting in soft tissue injury in the lower limbs. To prevent such injuries, functional training of the hamstrings requires intensive eccentric contractions. Additionally, hamstring function should be tested in relation to quadricep function at different contraction speeds. Biology Hemi-laryngeal Setup for Studying Vocal Fold Vibration in Three Dimensions Christian T. Herbst1,2, Vit Hampala1, Maxime Garcia2,3, Riccardo Hofer2, Jan G. Svec1 1Voice Research Lab, Department of Biophysics, Faculty of Science, Palacky University Olomouc, 2Laboratory of Bio-Acoustics, Dept. of Cognitive Biology, University of Vienna, 3ENES Lab, NEURO-PSI,CNRS UMR 9197, Université Lyon/Saint-Etienne, France This paper introduces a protocol for the preparation of hemi-larynx specimens facilitating a multi-dimensional view of vocal fold vibration, in order to investigate various biophysical aspects of voice production in humans and non-human mammals. Cancer Research Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter Plates Viswanath Das1, Tomáš Fürst1, Soňa Gurská1, Petr Džubák1, Marián Hajdúch1 1Institute of Molecular and Translational Medicine, Palacky University in Olomouc The uneven loss of medium from microtiter plates affects the reproducibility of uniform multicellular tumor spheroid formation. Improving culture conditions to reduce significant medium loss will improve the reproducibility of spheroid formation and the results of spheroid-based assays using the liquid-overlay technique.
