Reprofit International Brno 1 article published in JoVE Developmental Biology Human Egg Maturity Assessment and Its Clinical Application Zuzana Holubcová1,2, Drahomíra Kyjovská1, Martina Martonová1, Darja Páralová1, Tereza Klenková1, Soňa Kloudová1 1Reprofit International, Clinic of Reproductive Medicine, 2Department of Histology and Embryology, Faculty of Medicine, Masaryk University We provide an outline of the clinical protocol for non-invasive assessment of human egg maturity using polarized light microscopy.