Institute of Molecular Genetics of the Czech Academy of Sciences 3 articles published in JoVE Behavior Behavioral Characterization of an Angelman Syndrome Mouse Model Agnieszka Kubik-Zahorodna1, Jan Prochazka1,2, Radislav Sedlacek1,2 1Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, 2Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the Czech Academy of Sciences This manuscript presents a set of highly reproducible behavioral tests to validate an Angelman syndrome mouse model. Biology Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy Srikant Ojha1, Jan Pribyl2, Simon Klimovic2,3, Daniel Hadraba4, Marketa Jirouskova1, Martin Gregor1 1Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, 2CEITEC MU, Masaryk University, 3Department of Biochemistry, Faculty of Science, Masaryk University, 4Laboratory of Biomathematics, Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences We present a protocol to measure the elastic moduli of collagen-rich areas in normal and diseased liver using atomic force microscopy. The simultaneous use of polarization microscopy provides high spatial precision for localizing collagen-rich areas in the liver sections. Biology Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro Katerina Korelova1, Marketa Jirouskova1, Lenka Sarnova1, Martin Gregor1 1Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences Presented here is a protocol for the isolation of mouse hepatocytes from adult mouse livers using a modified collagenase perfusion technique. Also described is the long-term culture of hepatocytes in a 3D collagen sandwich setting as well as immunolabeling of the cytoskeletal components to study bile canalicular formation and its response to treatment.
