Central European Institute of Technology 1 article published in JoVE Medicine DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis Simona Hankeova*1, Jakub Salplachta*2, Noemi Van Hul1, Michaela Kavkova2, Afshan Iqbal1, Tomas Zikmund2, Jozef Kaiser2, Emma R. Andersson1 1Karolinska Institutet, 2Central European Institute of Technology Double resin casting micro-computed tomography, or DUCT, enables visualization, digitalization, and segmentation of two tubular systems simultaneously to facilitate 3D analysis of organ architecture. DUCT combines ex vivo injection of two radiopaque resins followed by micro-computed tomography scanning and segmentation of the tomographic data.