German Primate Center Leibniz Institute for Primate Research (DPZ) 4 articles published in JoVE Developmental Biology Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification Lidiia Tynianskaia1, Nesil Eşiyok1, Wieland B. Huttner2, Michael Heide1,2 1German Primate Center, Leibniz Institute for Primate Research, 2Max Planck Institute of Molecular Cell Biology and Genetics The electroporation of primate cerebral organoids provides a precise and efficient approach to introduce transient genetic modification(s) into different progenitor types and neurons in a model system close to primate (patho)physiological neocortex development. This allows the study of neurodevelopmental and evolutionary processes and can also be applied for disease modeling. Medicine Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts Laura Diaz-Maue*1,2, Janna Steinebach*1, Michael Schwaerzle8,9, Stefan Luther1,3,4,6, Patrick Ruther8,9, Claudia Richter1,5,6,7 1Research Group Biomedical Physics, Max Planck Institute for Dynamics and Self-Organization, 2Research Electronics Department, Max Planck Institute for Dynamics and Self-Organization, 3Department of Pharmacology and Toxicology, University Medical Center Goettingen, 4Institute for Nonlinear Dynamics, Georg-August-University Goettingen, 5Department of Cardiology and Pneumology, University Medical Center Goettingen, 6German Center for Cardiovascular Research, DZHK e.V., partner site Goettingen, 7Laboratory Animal Science Unit, German Primate Center Leibniz Institute for Primate Research, 8Department of Microsystems Engineering (IMTEK), University of Freiburg, 9Cluster of Excellence BrainLinks-BrainTools, University of Freiburg This work reports a method for controlling the cardiac rhythm of intact murine hearts of transgenic channelrhodopsin-2 (ChR2) mice using local photostimulation with a micro-LED array and simultaneous optical mapping of epicardial membrane potential. Behavior Simultaneous Transcranial Alternating Current Stimulation and Functional Magnetic Resonance Imaging Kathleen A. Williams1, Yuranny Cabral-Calderin1,3, Carsten Schmidt-Samoa1, Christiane Anne Weinrich1,2, Peter Dechent1, Melanie Wilke1,3,4 1Department of Cognitive Neurology, University Medicine Goettingen, 2Department of Neurology, University Medicine Goettingen, 3German Primate Center, Leibniz Institute for Primate Research, 4DFG Center for Nanoscale Microscopy & Molecular Physiology of the Brain (CNMPB) Transcranial alternating current stimulation (tACS) is a promising tool for noninvasive investigation of brain oscillations, though its effects are not completely understood. This article describes a safe and reliable setup for applying tACS simultaneously with functional magnetic resonance imaging, which can increase understanding oscillatory brain function and effects of tACS. Behavior A Pressure Injection System for Investigating the Neuropharmacology of Information Processing in Awake Behaving Macaque Monkey Cortex Vera K. Veith1, Cliodhna Quigley1, Stefan Treue1,2 1Cognitive Neuroscience Laboratory, German Primate Center, 2Faculty of Biology and Psychology, Goettingen University Here, we show the pressure injection of neuropharmacological substances during single-cell recording in an awake, behaving macaque monkey. This procedure allows pharmacological manipulation in the direct vicinity of a cortical recording site.
