Qiagen GmbH 1 article published in JoVE Cancer Research Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation Martina Loibner1,2, Lisa Oberauner-Wappis1,2, Christian Viertler2, Daniel Groelz3, Kurt Zatloukal1,2 1Christian Doppler Laboratory for Biospecimen Research and Biobanking Technologies, Institute of Pathology, Medical University Graz, 2Institute of Pathology, Medical University Graz, 3Research and Development, Qiagen GmbH Fluorescence in-situ hybridization (FISH) is often required in combination with histopathology and molecular diagnostics for selection of therapy in personalized medicine. A novel non-cross-linking, formalin-free tissue fixative that allows high quality morphologic, molecular and FISH analyses from the same specimen by addition of a post-fixation step before FISH is presented.
