Deutsches Herzzentrum Berlin (DHZB) 4 articles published in JoVE Medicine Transcatheter Pulmonary Valve Replacement from Autologous Pericardium with a Self-Expandable Nitinol Stent in an Adult Sheep Model Yimeng Hao1,2, Xiaolin Sun1,2, Jonathan Frederik Sebastian Kiekenap1, Jasper Emeis1, Marvin Steitz2, Alexander Breitenstein-Attach2, Felix Berger1,2, Boris Schmitt1,2,3,4,5 1Department of Pediatric Cardiology and Congenital Heart Disease, Charité University Medicine Berlin, 2Department of Pediatric Cardiology and Congenital Heart Disease, Deutsches Herzzentrum Berlin, 3DZHK (German Centre for Cardiovascular Research) and BMBF (German Ministry of Education and Research), 4BIH (Berlin Institute of Health), 5BCRT (BIH Center of Regenerative Therapies) This study demonstrates the feasibility and safety of developing an autologous pulmonary valve for implantation at the native pulmonary valve position by using a self-expandable Nitinol stent in an adult sheep model. This is a step toward developing transcatheter pulmonary valve replacement for patients with right ventricular outflow tract dysfunction. Medicine Four-Dimensional Computed Tomography-Guided Valve Sizing for Transcatheter Pulmonary Valve Replacement Xiaolin Sun*1,2, Yimeng Hao*1,2, Jonathan Frederik Sebastian Kiekenap1, Jasper Emeis1, Marvin Steitz2, Alexander Breitenstein-Attach2, Felix Berger1,2, Boris Schmitt1,2,3 1Department of Pediatric Cardiology and Congenital Heart Disease, Charité University Medicine Berlin, 2Department of Pediatric Cardiology and Congenital Heart Disease, Deutsches Herzzentrum Berlin, 3DZHK (German Centre for Cardiovascular Research) and BMBF (German Ministry of Education and Research) This study assessed a new methodology with a straightened model generated from the four-dimensional cardiac computed tomography sequence to obtain the desired measurements for valve sizing in the application of transcatheter pulmonary valve replacement. Developmental Biology Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications Matthias Becker1,2, Janita A. Maring1,2, Barbara Oberwallner2, Benjamin Kappler2, Oliver Klein1,2, Volkmar Falk2,3,4,5, Christof Stamm2,3,4 1Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health This protocol describes the complete decellularization of human myocardium while preserving its extracellular matrix components. Further processing of the extracellular matrix results in the production of microparticles and a cytoprotective self-assembling hydrogel. Medicine Assessment of Cardiac Function and Myocardial Morphology Using Small Animal Look-locker Inversion Recovery (SALLI) MRI in Rats Sarah Jeuthe1, Darach O H-Ici1, Ulrich Kemnitz1, Thore Dietrich2, Bernhard Schnackenburg3, Felix Berger1, Titus Kuehne1, Daniel Messroghli1,2 1Congenital Heart Disease and Pediatric Cardiology, German Heart Institute Berlin, 2Internal Medicine - Cardiology, German Heart Institute Berlin, 3Philips Health Care Contrast enhanced cardiac magnetic resonance (CMR) imaging allows comprehensive in vivo assessment of the heart in small animal models of cardiovascular disease. Here we detail the procedures to perform CMR imaging, reconstruction and analysis using Small Animal Lock-Locker Inversion Recovery (SALLI) in rats.
