Medical Faculty Mannheim of the Heidelberg University 6 articles published in JoVE Biology A Modified Surgical Model of Hind Limb Ischemia in ApoE-/- Mice using a Miniature Incision Kaixuan Yan1,2, Jiaxing Zheng1,2, Frank G. Zöllner3,4, Kay Schwenke1, Prama Pallavi1,2, Michael Keese1,2 1Department of Surgery, Medical Faculty Manheim, Heidelberg University, 2European Center of Angioscience ECAS, Medical Faculty Manheim, Heidelberg University, 3Computer-Assisted Clinical Medicine, Mannheim Institute for Intelligent Systems in Medicine, Medical Faculty Mannheim, Heidelberg University, 4Cooperative Core Facility Animal Scanner ZI, Medical Faculty Mannheim, Heidelberg University This article demonstrates an efficient surgical approach to establish acute ischemia in mice with a small incision. This approach can be applied by most research groups without any laboratory upgrades. Chemistry 18F-Labeling of Radiotracers Functionalized with a Silicon Fluoride Acceptor (SiFA) for Positron Emission Tomography David Connolly*1, Justin J. Bailey*1, Harun Ilhan2,3, Peter Bartenstein2,3, Carmen Wängler4, Björn Wängler5, Melinda Wuest1, Frank Wuest1, Ralf Schirrmacher1 1Department of Oncology, University of Alberta, 2Department of Nuclear Medicine, University Hospital of Munich, 3ENETS Centre of Excellence, Interdisciplinary Center of Neuroendocrine Tumors of the GastroEnteroPancreatic System, LMU Munich, 4Biomedical Chemistry, Department of Clinical Radiology and Nuclear Medicine, Medical Faculty Mannheim of Heidelberg University, 5Molecular Imaging and Radiochemistry, Department of Clinical Radiology and Nuclear Medicine, Medical Faculty Mannheim of Heidelberg University The synthesis of fluorine-18 (18F) labeled radiopharmaceuticals for positron emission tomography typically requires months of experience. When incorporated into a radiotracer, the silicon-fluoride acceptor (SiFA) motif enables a simple 18F-labeling protocol that is independent of costly equipment and preparatory training, while reducing precursor quantity needed and utilizing milder reaction conditions. Developmental Biology Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells Olivia Krefft*1, Ammar Jabali*1, Vira Iefremova1, Philipp Koch1,2,3, Julia Ladewig1 1Institute of Reconstructive Neurobiology, University of Bonn, 2Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, 3Hector Institute for Translational Brain Research (HITBR gGmbH) Cerebral organoids represent a new model system to investigate early human brain development in vitro. This article provides the detailed methodology to efficiently generate homogeneous dorsal forebrain-type organoids from human induced pluripotent stem cells including critical characterization and validation steps. Medicine Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature Lucas Moritz Wiggenhauser1, Katharina Kohl2, Nadine Dietrich2, Hans-Peter Hammes2, Jens Kroll1 1Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim, Heidelberg University, 2V. Medical Clinic, Medical Faculty Mannheim, Heidelberg University Here, we discuss a method protocol which will allow an easy analysis of the adult tg(fli:EGFP) zebrafish retinal vasculature as a fast read-out in settings of long-term vascular pathologies linked to neoangiogenesis and structural changes. Cancer Research Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks Henning D. Popp1, Susanne Brendel1, Wolf-Karsten Hofmann1, Alice Fabarius1 1Department of Hematology and Oncology, Medical Faculty Mannheim of the Heidelberg University This manuscript provides a protocol for the analysis of DNA double-strand breaks by immunofluorescence microscopy of γH2AX and 53BP1. Medicine Transcutaneous Assessment of Renal Function in Conscious Rodents Zeneida Herrera Pérez1, Stefanie Weinfurter1, Norbert Gretz1 1Medical Research Center, Medical Faculty Mannheim, University of Heidelberg Determination of glomerular filtration rate (GFR) is the gold standard to assess overall kidney function. However, traditional procedures to measure this parameter are cumbersome and require a large investment of time. Here we describe a faster and minimally invasive method to determinate GFR transcutaneously.
