Niederrhein University of Applied Sciences 1 article published in JoVE Environment Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation Melanie Voigt1,2, Christina Savelsberg1, Martin Jaeger1 1Niederrhein University of Applied Sciences, 2University Duisburg-Essen We present a protocol for non-targeted analysis using time of flight mass spectrometry as a perfect tool to identify pharmaceuticals in waters. We demonstrate the application of UV irradiation for their elimination. Analysis involving irradiation, compound isolation, identification and kinetic modelling of the degradation profiles is illustrated.