TU-Dresden 2 articles published in JoVE Cancer Research A 3D Organotypic Melanoma Spheroid Skin Model Ines Müller1, Dagmar Kulms1 1Experimental Dermatology, Medical Faculty, TU-Dresden Here, we present a protocol to generate a 3D organotypic melanoma spheroid skin model that recapitulates both the architecture and multicellular complexity of an organ/tumor in vivo but at the same time accommodates systematic experimental intervention. Immunology and Infection Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS Percy Schröttner1, Florian Gunzer1, Jana Schüppel2, Wolfram W. Rudolph2 1Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät Carl Gustav Carus, TU Dresden, 2Institut für Virologie, Medizinische Fakultät Carl Gustav Carus, TU Dresden Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and molecular techniques (16S rRNA gene sequencing) permit the identification of rare bacterial pathogens in routine diagnostics. The goal of this protocol lies in the combination of both techniques which leads to more accurate and reliable data.