Friedrich Schiller University of Jena 7 articles published in JoVE Neuroscience Whole-Brain 3D Activation and Functional Connectivity Mapping in Mice using Transcranial Functional Ultrasound Imaging Adrien Bertolo*1, Mohamed Nouhoum*1, Silvia Cazzanelli*1, Jeremy Ferrier*3, Jean-Charles Mariani2, Andrea Kliewer4,2, Benoit Belliard1, Bruno-Félix Osmanski3, Thomas Deffieux*1, Sophie Pezet*1, Zsolt Lenkei*2, Mickael Tanter*1 1Physics for Medicine Paris, ESPCI Paris, INSERM, CNRS, PSL Research University, 2Institute of Psychiatry and Neurosciences of Paris, INSERM U1266, Université de Paris, 3Iconeus, 4Department of Pharmacology and Toxicology, Jena University Hospital - Friedrich Schiller University Jena This protocol describes the quantification of volumetric cerebral hemodynamic variations in the mouse brain using functional ultrasound (fUS). Procedures for 3D functional activation map following sensory stimulation as well as resting-state functional connectivity are provided as illustrative examples, in anesthetized and awake mice. Genetics Monitoring Spatial Segregation in Surface Colonizing Microbial Populations Theresa Hölscher1, Anna Dragoš1, Ramses Gallegos-Monterrosa1, Marivic Martin1, Eisha Mhatre1, Anne Richter1, Ákos T. Kovács1 1Terrestrial Biofilms Group, Institute of Microbiology, Friedrich Schiller University, Jena The role of assortment (spatial segregation) in evolutionary scenarios can be examined using simple microbial systems in the laboratory that allow controlled adjustment of spatial distribution. By modifying the founder cell density, various assortment levels can be visualized using fluorescently labelled bacterial strains in the colony biofilms of Bacillus subtilis. Chemistry Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue Julia Greiser1, Tobias Niksch1, Wolfgang Weigand2, Martin Freesmeyer1 1Clinic of Nuclear Medicine, University Hospital Jena, 2Friedrich Schiller University A procedure for the isolation of EOB-DTPA and subsequent complexation with natural Ga(III) and 68Ga is presented herein, as well as a thorough analysis of all compounds and investigations on labeling efficiency, in vitro stability and the n-octanol/water distribution coefficient of the radiolabeled complex. Developmental Biology Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning Birgit Perner1, Danny Schnerwitzki1, Michael Graf1,3, Christoph Englert1,2 1Molecular Genetics, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), 2Faculty of Biology and Pharmacy, Friedrich Schiller University of Jena, 3Carl Zeiss Microscopy GmbH The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift. Bioengineering Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging Felista L. Tansi*1, Ronny Rüger*2, Markus Rabenhold2, Frank Steiniger3, Alfred Fahr2, Ingrid Hilger1 1Experimental Radiology, Institute of Diagnostic and Interventional Radiology I, Jena University Hospital, 2Department of Pharmaceutical Technology, Friedrich-Schiller-University Jena, 3Center for Electron Microscopy, Jena University Hospital The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity. Immunology and Infection Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection Marten B. Maeß1, Berith Wittig1, Stefan Lorkowski1 1Institute of Nutrition, Friedrich Schiller University Jena This protocol presents an efficient and reliable method to transfect human THP-1 macrophages with siRNA or plasmid DNA by electroporation with high transfection efficiency while maintaining high cell vitality and full macrophage capacity for differentiation and polarization. Neuroscience In Vivo Electrophysiological Measurements on Mouse Sciatic Nerves Alexander Schulz1, Christian Walther2, Helen Morrison1, Reinhard Bauer3 1Leibniz Institute for Age Research, Fritz Lipmann Institute, 2Friedrich Schiller University Jena, 3Institute of Molecular Cell Biology & Center for Sepsis Control and Care (CSCC) Jena University Hospital, Friedrich Schiller University Jena Measurements of nerve conduction properties in vivo exemplify a powerful tool to characterize various animal models of neuromuscular diseases. Here, we present an easy and reliable protocol by which electrophysiological analysis on sciatic nerves of anesthetized mice can be performed.