Carl Zeiss Microscopy GmbH 1 article published in JoVE Developmental Biology Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning Birgit Perner1, Danny Schnerwitzki1, Michael Graf1,3, Christoph Englert1,2 1Molecular Genetics, Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), 2Faculty of Biology and Pharmacy, Friedrich Schiller University of Jena, 3Carl Zeiss Microscopy GmbH The method described here allows time-lapse analysis of organ development in zebrafish embryos by using a fluorescence dissecting microscope capable of performing optical sectioning and simple strategies of readjustment to correct focal and planar drift.