Technical University of Dortmund 1 article published in JoVE Developmental Biology Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation Vaibhav Shinde*1, Stefanie Klima*2, Perumal Srinivasan Sureshkumar1, Kesavan Meganathan1, Smita Jagtap1, Eugen Rempel3, Jörg Rahnenführer3, Jan Georg Hengstler4, Tanja Waldmann2, Jürgen Hescheler1, Marcel Leist*2, Agapios Sachinidis*1 1Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, 2Department of Biology, University of Konstanz, 3Department of Statistics, Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund The protocols describe two in vitro developmental toxicity test systems (UKK and UKN1) based on human embryonic stem cells and transcriptome studies. The test systems predict human developmental toxicity hazard, and may contribute to reduce animal studies, costs and the time required for chemical safety testing.
