University of Regensburg 5 articles published in JoVE Engineering Evaluating Usability Aspects of a Mixed Reality Solution for Immersive Analytics in Industry 4.0 Scenarios Burkhard Hoppenstedt1, Thomas Probst2, Manfred Reichert1, Winfried Schlee3, Klaus Kammerer1, Myra Spiliopoulou4, Johannes Schobel1, Michael Winter1, Anna Felnhofer5, Oswald D. Kothgassner6, Rüdiger Pryss7 1Institute of Databases and Information Systems, Ulm University, 2Department for Psychotherapy and Biopsychosocial Health, Danube University Krems, 3Department of Psychiatry and Psychotherapy, University of Regensburg, 4Faculty of Computer Science, Otto von Guericke University Magdeburg, 5Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, 6Department of Child and Adolescent Psychiatry, Medical University of Vienna, 7Institute of Clinical Epidemiology and Biometry, University of Würzburg This protocol delineates the technical setting of a developed mixed reality application that is used for immersive analytics. Based on this, measures are presented, which were used in a study to gain insights into usability aspects of the developed technical solution. Biochemistry Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays Judith A. Stolwijk1, Anne-Kathrin Mildner1, Christian Kade1, Michael Skiba1, Guenther Bernhardt2, Armin Buschauer2, Harald Huebner3, Peter Gmeiner3, Joachim Wegener1,4 1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution. Genetics Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins Isabell-Christin Fiedler1, Arne Weiberg2, Karina van der Linde1 1Department of Biology, Regensburg University, 2Department of Biology, Ludwig-Maximilians University of Munich This work describes the cloning of an Ustilago maydis Trojan horse strain for the in situ delivery of secreted maize proteins into three different types of maize tissues. Biology The Mouse Isolated Perfused Kidney Technique Jan Czogalla1, Frank Schweda2, Johannes Loffing1 1Institute of Anatomy, Swiss National Centre of Competence in Research Kidney, University of Zürich, 2Department of Physiology, University of Regensburg The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail. Immunology and Infection Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells Christian Schütz1, Martin Fleck2,3, Jonathan P. Schneck1, Mathias Oelke1 1Department of Pathology, Institute of Cell Engineering, Johns Hopkins University, 2Department of Internal Medicine I, University of Regensburg, 3Department of Rheumatology, Asklepios Medical Center Guidelines are presented for the generation of killer artificial antigen presenting cells, KaAPC, an efficient tool for in vitro depletion of human antigen-specific T cells and an alternative solution to cellular immunotherapy for the treatment of T cell-mediated autoimmune diseases in an antigen-specific fashion without compromising the remaining immune system.
