University of Lubeck 4 articles published in JoVE Medicine Development of a Noninvasive, Laser-Assisted Experimental Model of Corneal Endothelial Cell Loss Annekatrin Holzhey*1, Svenja Sonntag*2, Johannes Rendenbach1, Justus S. Ernesti1, Vinodh Kakkassery2, Salvatore Grisanti2, Fred Reinholz3, Sebastian Freidank3, Alfred Vogel3, Mahdy Ranjbar1,2 1Laboratory for Angiogenesis and Ocular Cell Transplantation, University of Lübeck, 2Department of Ophthalmology, University of Lübeck, 3Institute of Biomedical Optics, University of Lübeck Here, we present a protocol to detach corneal endothelial cells (CEC) from Descemet’s membrane (DM) using a neodymium:YAG (Nd:YAG) laser as an ex vivo disease model for bullous keratopathy (BK). Engineering Robotic Sensing and Stimuli Provision for Guided Plant Growth Mostafa Wahby1, Mary Katherine Heinrich1,5, Daniel Nicolas Hofstadler2, Julian Petzold1, Igor Kuksin3, Payam Zahadat2,4, Thomas Schmickl2, Phil Ayres5, Heiko Hamann1 1Institute of Computer Engineering, University of Lübeck, 2Institute of Biology, Artificial Life Lab, University of Graz, 3Cybertronica UG, 4Department of Computer Science, IT University of Copenhagen, 5Centre for IT and Architecture, Royal Danish Academy Distributed robot nodes provide sequences of blue light stimuli to steer the growth trajectories of climbing plants. By activating natural phototropism, the robots guide the plants through binary left-right decisions, growing them into predefined patterns that by contrast are not possible when the robots are dormant. Immunology and Infection Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain Claudia Pösel*1, Karoline Möller*1, Johannes Boltze1,2, Daniel-Christoph Wagner1, Gesa Weise1,3 1Ischemia Research Unit, Fraunhofer Institute for Cell Therapy and Immunology, 2Fraunhofer Research Institution for Marine Biotechnology, University of Lubeck, 3Department of Neurology, University of Leipzig Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping. Bioengineering A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo Thilo L. Schenck1, Myra N. Chávez1, Alexandru P. Condurache2, Ursula Hopfner1, Farid Rezaeian3, Hans-Günther Machens1, José T. Egaña1,4 1Department of Plastic Surgery and Hand Surgery, University Hospital rechts der Isar, Technische Universität München, 2Institute for Signal Processing, University of Lübeck, 3Department of Plastic Surgery and Hand Surgery, University Hospital Zürich, 4FONDAP Center for Genome Regulation, Facultad de Ciencias, Universidad de Chile Vascularization is key to approaches in successful tissue engineering. Therefore, reliable technologies are required to evaluate the development of vascular networks in tissue-constructs. Here we present a simple and cost-effective method to visualize and quantify vascularization in vivo.
