Max-Planck Institute for Heart and Lung Research 2 articles published in JoVE Biochemistry Identification of Functional Protein Regions Through Chimeric Protein Construction Juan M. Adrian-Segarra1, Holger Lörchner1,2, Thomas Braun1,2, Jochen Pöling1,2 1Department of Cardiac Development and Remodelling, Max Planck Institute for Heart and Lung Research, 2Partner site Rhein-Main, German Centre for Cardiovascular Research (DZHK) Structurally related proteins frequently exert distinct biological functions. The exchange of equivalent regions of these proteins in order to create chimeric proteins constitutes an innovative approach to identify critical protein regions that are responsible for their functional divergence. Biology Recovery of Adult Zebrafish Hearts for High-throughput Applications Rima Arnaout1, Sven Reischauer2,3, Didier Y.R. Stainier2,3 1Cardiovascular Research Institute and Division of Cardiology, University of California San Francisco, 2Department for Biochemistry and Biophysics, University of California San Francisco, 3Max-Planck Institute for Heart and Lung Research Use of zebrafish for cardiovascular research is expanding towards research on adult hearts. For these applications, quick and simple isolation of cardiac tissues is key to avoid post-mortem changes and to obtain an adequate number of samples. Here, we describe a fast and reproducible method for dissecting adult zebrafish hearts.
