DZHK (German Center for Cardiovascular Research) Partner site Munich Heart Alliance 5 articles published in JoVE Medicine Real-Time Electrocardiogram Monitoring During Treadmill Training in Mice Philipp Tomsits*1,2,4, Aparna Sharma Chivukula*1,2, Kavi Raj Chataut*1,2, Agus Simahendra3, Ludwig T. Weckbach1,2,3, Stefan Brunner1, Sebastian Clauss1,2,4 1Department of Medicine I, University Hospital Munich, Ludwig-Maximilians University Munich (LMU), 2DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), 3Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-University, 4Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians University Munich (LMU) Electrocardiogram (ECG) is the key variable to understanding cardiac electrophysiology. Physical exercise has beneficial effects but may also be harmful in the context of cardiovascular diseases. This manuscript provides a method of recording real-time ECG during exercise, which can serve to investigate its effects on cardiac electrophysiology in mice. Medicine Combining 3D-Printing and Electrospinning to Manufacture Biomimetic Heart Valve Leaflets Benedikt Freystetter1, Maximilian Grab1,2, Linda Grefen1, Lara Bischof1, Lorenz Isert3, Petra Mela2, Deon Bezuidenhout4, Christian Hagl1,5, Nikolaus Thierfelder1 1Department of Cardiac Surgery, Ludwig Maximilians University Munich, 2Chair of Medical Materials and Implants, Technical University Munich, 3Faculty for Chemistry and Pharmacy, Ludwig Maximilians University Munich, 4Cardiovascular Research Unit, University of Cape Town, 5DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance The presented method offers an innovative way for engineering biomimetic fiber structures in three-dimensional (3D) scaffolds (e.g., heart valve leaflets). 3D-printed, conductive geometries were used to determine shape and dimensions. Fiber orientation and characteristics were individually adjustable for each layer. Multiple samples could be manufactured in one setup. Medicine Analyzing Long-Term Electrocardiography Recordings to Detect Arrhythmias in Mice Philipp Tomsits1,2,3, Kavi Raj Chataut1,2, Aparna Sharma Chivukula1,2, Li Mo1,2, Ruibing Xia1,2, Dominik Schüttler1,2,3, Sebastian Clauss1,2,3 1Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), 2DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), 3Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University Munich (LMU) Here we present a step-by-step protocol for a semiautomated approach to analyze murine long-term electrocardiography (ECG) data for basic ECG parameters and common arrhythmias. Data are obtained by implantable telemetry transmitters in living and awake mice and analyzed using Ponemah and its analysis modules. Medicine Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node Ruibing Xia1,2,3, Simone Loy1, Stefan Kääb1,3, Anna Titova1, Christian Schulz1,2,3, Steffen Massberg1,2,3, Sebastian Clauss1,2,3 1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA) The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts. Biology Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles Harald Lahm*1, Stefanie A. Doppler*1, Martina Dreßen1, Klaudia Adamczyk1, Marcus-André Deutsch1, Hanna Ulrich2, Matthias Schiemann2,3, Rüdiger Lange1,4, Markus Krane1,4 1Department of Cardiovascular Surgery, German Heart Center Munich, Technische Universität München, 2Institute for Medical Microbiology, Immunology, and Hygiene, Technische Universität München, 3Clinical Cooperation Groups: "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich (Neuhererg), Technische Universität München, 4DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance This manuscript describes a novel technique which allows the detection of intracellular gene expression after endocytosis of fluorescence-labeled nanoparticles directly in live cells. The method does not require manipulation of the cells and is not restricted with respect to the target gene or species.
