Max Planck Institute of Molecular Physiology 1 article published in JoVE Cancer Research How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases Mercedes Rodriguez-Teja1, Claudia Breit2, Mitchell Clarke3, Kamil Talar3, Kai Wang3, Mohammad A. Mohammad3, Sage Pickwell3, Guillermina Etchandy1, Graeme J. Stasiuk3, Justin Sturge3 1Departamento de Genética, Facultad de Medicina, Universidad de la República (UDELAR), 2Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, 3School of Biological, Biomedical & Environmental Sciences, University of Hull Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological relevance.
