La Vision Biotec GmbH 1 article published in JoVE Immunology and Infection Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers Zoltan Cseresnyes*1,2, Laura Oehme*3, Volker Andresen4, Anje Sporbert2, Anja E. Hauser*3,5, Raluca Niesner*1 1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine High-resolution intravital imaging with enhanced contrast up to 120 µm depth in lymph nodes of adult mice is achieved by spatially modulating the excitation pattern of a multi-focal two-photon microscope. In 100 µm depth we measured resolutions of 487 nm (lateral) and 551 nm (axial), thus circumventing scattering and diffraction limits.
