Center for Systems Biology Dresden 1 article published in JoVE Developmental Biology Analysis of Actomyosin Dynamics at Local Cellular and Tissue Scales Using Time-lapse Movies of Cultured Drosophila Egg Chambers Ivana Viktorinová1,2, Robert Haase*1,2, Tobias Pietzsch*1,2, Ian Henry1,2, Pavel Tomancak1,2 1Max Planck Institute of Molecular Cell Biology and Genetics, 2Center for Systems Biology Dresden This protocol provides a Fiji-based, user-friendly methodology along with straightforward instructions explaining how to reliably analyze actomyosin behavior in individual cells and curved epithelial tissues. No programming skills are required to follow the tutorial; all steps are performed in a semi-interactive manner using the graphical user interface of Fiji and associated plugins.
