Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT 1 article published in JoVE Biochemistry Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays Judith A. Stolwijk1, Anne-Kathrin Mildner1, Christian Kade1, Michael Skiba1, Guenther Bernhardt2, Armin Buschauer2, Harald Huebner3, Peter Gmeiner3, Joachim Wegener1,4 1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.
