Technische Universitat Braunschweig 2 articles published in JoVE Neuroscience In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal Paul Schramm1, Florian Hetsch2, Jochen C. Meier3, Reinhard W. Köster1 1Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Institute of Pathophysiology, University Medical Center of the Johannes Gutenberg University, 3Cell Physiology, Zoological institute, Technische Universität Braunschweig Here we present a method to image the zebrafish embryonic brain in vivo upto larval and juvenile stages. This microinvasive procedure, adapted from electrophysiological approaches, provides access to cellular and subcellular details of mature neuron and can be combined with optogenetics and neuropharmacological studies for characterizing brain function and drug intervention. Developmental Biology Development of a Larval Zebrafish Infection Model for Clostridioides difficile Junkai Li1, Can M. Ünal2, Kazuhiko Namikawa1, Michael Steinert3,4,5, Reinhard W. Köster1 1Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Department of Molecular Biotechnology, Turkish-German University, 3Institut für Mikrobiologie, Technische Universität Braunschweig, 4Braunschweig Integrated Centre of Systems Biology, 5Helmholtz Centre for Infection Research Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.
