Charite-Universitatsmedizin Berlin, Freie Universitat Berlin, Humbolt-Universitat zu Berlin, and Ber 3 articles published in JoVE Medicine Surfactant Depletion Combined with Injurious Ventilation Results in a Reproducible Model of the Acute Respiratory Distress Syndrome (ARDS) Martin Russ*1, Emilia Boerger*1, Philip von Platen2, Roland C. E. Francis1, Mahdi Taher1, Willehad Boemke1, Burkhard Lachmann1, Steffen Leonhardt2, Philipp A. Pickerodt1 1Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt Universität zu Berlin and Berlin Institute of Health Department of Anesthesiology and Intensive Care Medicine, Campus Charité Mitte and Campus Virchow-Klinikum, 2Chair for Medical Information Technology, RWTH Aachen University A combination of surfactant washout using 0.9% saline (35 mL/kg body weight, 37 °C) and high tidal volume ventilation with low PEEP to cause moderate ventilator induced lung injury (VILI) results in experimental acute respiratory distress syndrome (ARDS). This method provides a model of lung injury with low/limited recruitability to study the effect of various ventilation strategies for extended periods. Immunology and Infection In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time Xue D. Manz1, Hugo J. Albers2,3, Petr Symersky4, Jurjan Aman1, Andries D. van der Meer3, Harm Jan Bogaard1, Robert Szulcek1,5,6 1Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group, University of Twente, 3Applied Stem Cell Technologies Group, University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center, 5Institute of Physiology, Charité-Universitätsmedizin, 6German Heart Center We present an in vitro vascular disease model to investigate whole blood interactions with patient-derived endothelium. This system allows the study of thrombogenic properties of primary endothelial cells under various circumstances. The method is especially suited to evaluate in situ thrombogenicity and anticoagulation therapy during different phases of coagulation. Neuroscience Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings Larissa Kraus1, Laura Monni1,2, Ulf C. Schneider3, Julia Onken3, Philipp Spindler3, Martin Holtkamp1, Pawel Fidzinski1,2 1Department of Neurology with Experimental Neurology, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2NeuroCure Cluster of Excellence, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3Department of Neurosurgery, Charité-Universitätsmedizin Berlin, Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health The presented protocol describes the transport and preparation of resected human hippocampal tissue with the ultimate goal to use vital brain slices as a preclinical evaluation tool for potential antiepileptic substances.
