Partner Site Heidelberg-Mannheim 1 article published in JoVE Developmental Biology Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils Soah Lee*1,2,3, Huaxiao Yang*1,2,3, Caressa Chen*1,2,3, Sneha Venkatraman1,2,3, Adrija Darsha1,2,3, Sean M. Wu1,2,3, Joseph C. Wu1,2,3, Timon Seeger1,2,3,4,5 1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 4Department of Medicine III, University Hospital Heidelberg, 5German Centre for Cardiovascular Research (DZHK), Partner Site Heidelberg/Mannheim This protocol introduces a lithography-free micropatterning method that is simple and accessible to those with a limited bioengineering background. This method utilizes customized laser-cut stencils to micropattern extracellular matrix proteins in a shape of interest for modulating cell morphologies. The procedure for micropatterning is demonstrated using induced pluripotent stem cell derived cardiomyocytes.
