Julius-Maximilian University Wuerzburg 1 article published in JoVE Biochemistry Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells Katherina Hemmen*1, Susobhan Choudhury*1, Mike Friedrich1, Johannes Balkenhol1, Felix Knote1, Martin J. Lohse2, Katrin G. Heinze1 1Rudolf Virchow Center for Integrative and Translation Bioimaging, Julius-Maximilian University Wuerzburg, 2Max Delbrück Center for Molecular Medicine, Berlin We present an experimental protocol and data analysis workflow to perform live cell dual-color fluorescence cross correlation spectroscopy (FCCS) combined with Förster Resonance Energy transfer (FRET) to study membrane receptor dynamics in live cells using modern fluorescence labeling techniques.
