Research Laboratory for Pediatric Surgery 1 article published in JoVE Medicine Extrahepatic Bile Duct and Gall Bladder Dissection in Nine-Day-Old Mouse Neonates Hans Christian Schmidt1,2,3, Johanna Hagens1,2,3, Pauline Schuppert1,2, Clara Philippi1,2, Konrad Reinshagen1,2, Christian Tomuschat1,2 1Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, 2Research Laboratory for Pediatric Surgery, 3Research Animal Facility, University Medical Center Hamburg-Eppendorf For the observation of murine neonatal bile duct disorders, an intact bile duct and efficient preparation are required. Therefore, a new approach for isolating the entire extrahepatic bile duct system in murine neonates was successfully developed while maintaining the integrity of the bile duct.