University of Valencia

5 articles published in JoVE

• Environment

  
  Data Collection on Marine Litter Ingestion in Sea Turtles and Thresholds for Good Environmental Status Marco Matiddi¹, Giuseppe A. deLucia², Cecilia Silvestri¹, Gaëlle Darmon³, Jesús Tomás⁴, Christopher K. Pham⁵, Andrea Camedda², Frederic Vandeperre^5,6, Françoise Claro⁷, Yakup Kaska⁸, Helen Kaberi⁹, Ohiana Revuelta⁴, Raffaella Piermarini¹, Roberto Daffina¹, Marco Pisapia¹, Daniela Genta¹, Doğan Sözbilen⁸, Mohamed N. Bradai¹⁰, Yasmina Rodríguez⁵, Delphine Gambaiani³, Catherine Tsangaris⁹, Olfa Chaieb¹⁰, Judicaëlle Moussier⁷, Ana L. Loza¹¹, Claude Miaud³, INDICIT consortium, ¹Italian National Institute for Environmental Protection and Research (ISPRA), ²Institute for Coastal Marine Environment-National Research Council (IAMC-CNR), ³EPHE, PSL Research University, UMR 5175 CE3FE, CNRS, UM, Univ P. Valery, SupAgro, IRD, INRA, Biogéographie et Écologie des Vertébrés, ⁴Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, ⁵Departamento de Oceanografia e Pescas, Instituto do Mar/Okeanos, Universidade dos Açores, ⁶MARE - Marine and Environmental Sciences Centre, Universidade dos Açores, ⁷ The protocol focuses on the collection of sea turtle samples, describing all the steps from the animal recovery and necropsy to the classification and quantification of ingested marine litter. Moreover, the representative results show how to use the collected data to elaborate the possible thresholds for Good Environmental Status.

• Biology

  
  Fish Sperm Assessment Using Software and Cooling Devices Carina Caldeira¹, Carles Soler^{1,2 1}PROiSER R+D S.L., ²Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia The present protocol describes a procedure of fish sperm assessment using computer-assisted sperm analysis and cooling devices. The software gives a rapid, accurate and quantitative analysis of fish sperm quality based on spermatozoa motility, which can be a useful tool in aquaculture to improve reproduction success.

• Behavior

  
  Reinstatement of Drug-seeking in Mice Using the Conditioned Place Preference Paradigm M. Carmen Blanco-Gandía¹, María A. Aguilar¹, José Miñarro¹, Marta Rodríguez-Arias^{1 1}Unidad de Investigación Psicobiología de las Drogodependencias, Departamento de Psicobiología, Facultad de Psicología, Universitat de València This protocol describes the Conditioned Place Preference (CPP) as a model of relapse. This procedure permits the measurement of relapse in laboratory animals, considering the impact of drug-associated environmental cues as craving and relapse in abstaining addicts is currently the focus of drug-abuse treatment programs.

• Medicine

  
  An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment Óscar Julián Arias-Mutis^1,2,3, Patricia Genovés^1,2,3, Conrado J. Calvo^1,2,4, Ana Díaz⁵, Germán Parra^2,3, Luis Such-Miquel⁶, Luis Such², Antonio Alberola², Francisco Javier Chorro^1,3, Manuel Zarzoso^{6 1}CIBERCV, Instituto de Salud Carlos III, ²Department of Physiology, Universitat de València, ³INCLIVA, ⁴Department of Electronic Engineering, Universidad Politécnica de Valencia, ⁵UCIM, Universitat de València, ⁶Department of Physiotherapy, Universitat de València We describe methods to develop an experimental model of diet-induced metabolic syndrome (MetS) in rabbits using a high-fat, high-sucrose diet. Animals developed central obesity, mild hypertension, pre-diabetes, and dyslipidemia, thus reproducing the main components of human MetS. This chronic model will allow acquisition of knowledge underlying mechanisms of disease progression.

• Biology

  
  Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles Estela Selma-Soriano^1,2,3, Rubén Artero^1,2,3, Beatriz Llamusi^{1,2,3 1}Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED), University of Valencia, ²Incliva Health Research Institute, ³Joint unit CIPF-Incliva We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.