Universidad de Malaga (UMA) 5 articles published in JoVE Biology Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue José S. Rufián*1, Nieves López-Pagán*1, Javier Ruiz-Albert1, Carmen R. Beuzón1 1Depto. Biología Celular, Genética y Fisiología, Instituto de Hortofruticultura Subtropical y Mediterránea, Campus de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC) The present protocol describes a method that allows single-cell gene expression analysis on Pseudomonas syringae populations grown within the plant apoplast. Medicine Basophil Activation Test for Allergy Diagnosis Inmaculada Doña*1,2, Adriana Ariza*2, Tahia D. Fernández2,3, María J. Torres1,2,4,5 1Allergy Unit, Hospital Regional Universitario de Málaga, 2Allergy Research Group, Instituto de Investigación Biomédica de Málaga-IBIMA, 3Universidad de Málaga, Departamento de Biología celular, Genética y Fisiología, Universidad de Málaga, 4Departamento de Medicina, Universidad de Málaga, 5Nanostructures for Diagnosing and Treatment of Allergic Diseases Laboratory, Andalusian Center for Nanomedicine and Biotechnology-BIONAND The basophil activation test is a complementary in vitro diagnostic test for the evaluation of IgE-mediated allergic reactions based on the detection of basophil activation in the presence of a specific stimulus through the measure of activation markers by flow cytometry. Behavior Assessing Dyslexia at Six Year of Age María-José González-Valenzuela1, isaías Martín-Ruiz1 1Department of Developmental and Educational Psychology, University of Málaga This research sets out a proposed protocol for the identification of dyslexia. The protocol is based on diagnostic and response to intervention models. The proposal involves using structured interviews and standardized tests for the assessment of reading and writing performance and determinant factors. Bioengineering Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation Ignasi Casanellas1,2, Anna Lagunas3,1, Iro Tsintzou1, Yolanda Vida4,5, Daniel Collado4,5, Ezequiel Pérez-Inestrosa4,5, Cristina Rodríguez-Pereira6, Joana Magalhaes3,6, Pau Gorostiza1,3,7, José A. Andrades8,3, José Becerra8,3,5, Josep Samitier1,3,2 1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 2Department of Engineering Electronics, University of Barcelona (UB), 3Networking Biomedical Research Center (CIBER), 4Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), 5Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, 6Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), 7Institució Catalana de Recerca i Estudis Avançats (ICREA), 8Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology, Universidad de Málaga (UMA) A method to obtain dendrimer-based uneven nanopatterns that permit the nanoscale control of local arginine-glycine-aspartic acid (RGD) surface density is described and applied for the study of cell adhesion and chondrogenic differentiation. Genetics Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs Scott E. Gold1, Zahi Paz2, María D. García-Pedrajas3, Anthony E. Glenn1 1Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, 2Hazera Seeds LTD, Brurim, 3Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora” Gene deletion mutants generated through homologous recombination are the gold standard for gene function studies. The OSCAR (One Step Construction of Agrobacterium-Recombination-ready-plasmids) method for rapid generation of deletion constructs is described. Agrobacterium mediated fungal transformation follows. Finally, a PCR based confirmation method of gene deletions in fungal transformants is presented.
