INCLIVA 2 articles published in JoVE Biology Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis Jessica Martinez1, Viviana Bisbal2, Nerea Marin2, Antonio Cano1,3,4, Raul Gómez1 1Instituto de Investigación Sanitaria, 2Unidad de Animalario del Centro de Investigación Príncipe Felipe (CIPF), 3Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, 4Servicio de Obstetricia y Ginecología, Hospital Clínico Universitario de Valencia Here, we present a protocol for live-imaging of fluorescently labeled human endometrial fragments grafted in mice. The method allows studying the effects of drugs of choice on endometriotic lesion size through monitoring and quantification of fluorescence emitted by the fluorescent reporter on real time Medicine An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment Óscar Julián Arias-Mutis1,2,3, Patricia Genovés1,2,3, Conrado J. Calvo1,2,4, Ana Díaz5, Germán Parra2,3, Luis Such-Miquel6, Luis Such2, Antonio Alberola2, Francisco Javier Chorro1,3, Manuel Zarzoso6 1CIBERCV, Instituto de Salud Carlos III, 2Department of Physiology, Universitat de València, 3INCLIVA, 4Department of Electronic Engineering, Universidad Politécnica de Valencia, 5UCIM, Universitat de València, 6Department of Physiotherapy, Universitat de València We describe methods to develop an experimental model of diet-induced metabolic syndrome (MetS) in rabbits using a high-fat, high-sucrose diet. Animals developed central obesity, mild hypertension, pre-diabetes, and dyslipidemia, thus reproducing the main components of human MetS. This chronic model will allow acquisition of knowledge underlying mechanisms of disease progression.
