National Cancer Research Center (CNIO) 2 articles published in JoVE Cancer Research Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration Maria Riedel1, Martin F. Berthelsen1, Latifa Bakiri2, Erwin F. Wagner2, Martin K. Thomsen1 1Department of Clinical Medicine, Aarhus University, 2GDD, Cancer Cell Biology Program, National Cancer Research Center (CNIO) This protocol describes a newly established method for virus delivery to the murine prostate. Using either CRISPR/Cas9 technology, gene overexpression, or Cre recombinase delivery, the technique allows orthotopic alteration of gene expression and implements a novel mouse model for prostate cancer. Developmental Biology Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects Eva Porlan1, Beatriz Martí-Prado2,3, Antonella Consiglio4,5, Isabel Fariñas2,3 1Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), 2Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 3Departmento de Biologìa Celular, Universidad de Valencia, 4Institut de Biomedicina de la Universitat de Barcelona (IBUB), 5Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit, University of Brescia Here we describe a procedure based on the use of lentiviral particles for the long-term genetic modification of neural stem cells and/or their adjacent ependymal cells in the adult ventricular-subventricular neurogenic niche which allows the separate analysis of cell autonomous and non-autonomous, niche-dependent effects on neural stem cells.
