Institute for Research in Biomedicine (IRB Barcelona) 1 article published in JoVE Medicine Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies Enza Lonardo1,2, Michele Cioffi1, Patricia Sancho1,3, Shanthini Crusz3, Christopher Heeschen1,3 1Stem Cells & Cancer Group, Molecular Pathology Program, Spanish National Cancer Research Center, 2Institute for Research in Biomedicine (IRB Barcelona), 3Center for Stem Cells in Cancer & Ageing, Barts Cancer Institute, Queen Mary University of London Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.
