Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) 1 article published in JoVE Bioengineering Sandwich-like Microenvironments to Harness Cell/Material Interactions José Ballester-Beltrán1,2, Myriam Lebourg1,3, Manuel Salmerón-Sánchez2 1Center for Biomaterials and Tissue Engineering, Universitat Politècnica de València, 2Division of Biomedical Engineering, School of Engineering, University of Glasgow, 3Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.
