Instituto de Investigaciones Agrarias Finca La Orden 1 article published in JoVE Developmental Biology Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping Brenda I. Guerrero1,2, Ma. Engracia Guerra3, Javier Rodrigo1,2 1Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), 2(CITA-Universidad de Zaragoza), Instituto Agroalimentario de Aragón - IA2, 3Departamento de Hortofruticultura, Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), Instituto de Investigaciones Agrarias Finca La Orden A methodology for the determination of pollination requirements in Japanese plum-type hybrids is described, which combines field- and laboratory-pollinations and observations of pollen tubes under the fluorescence microscopy with the identification of S-genotypes by PCR and the monitoring of flowering for the selection of pollinizers.
