Beamline 09 MISTRAL 1 article published in JoVE Biology Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells Nina Vyas*1, Nina Perry*1, Chidinma A. Okolo1, Ilias Kounatidis1, Thomas M. Fish1, Kamal L. Nahas1,2, Archana Jadhav1, Mohamed A. Koronfel1, Johannes Groen3, Eva Pereiro3, Ian M. Dobbie4, Maria Harkiolaki1 1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells.
