Universidad Pablo de Olavide 1 article published in JoVE Biology Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays Juan Diego Hernández-Camacho1,2, Cristina Vicente-García1, Ana Sánchez-Cuesta1,2, Daniel J. M. Fernandez-Ayala1,2, Jaime J. Carvajal1, Plácido Navas1,2 1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA, Universidad Pablo de Olavide, 2CIBERER, Instituto de Salud Carlos III Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.
